Anti-PDGF B Antibody (A286019)

Name: Anti-PDGF B Antibody
Brand: Antibodies.com
SKU: A286019
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to PDGF B for ELISA, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-PDGF B Antibody

Description

Goat polyclonal antibody to PDGF B.

Applications

ELISA, IF, Flow Cytometry

Dilutions

ELISA: 1:128,000, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the C terminal region of human PDGF B.

Sequence

C-KHRKFKHTHDKT

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Becaplermin, c sis, FLJ12858, Oncogene SIS, PDGF 2, PDGF B chain, PDGF Beta, PDGF subunit B, PDGF-2, PDGF2, Pdgfb, PDGFB_HUMAN, Platelet derived growth factor 2, Platelet derived growth factor B chain, Platelet derived growth factor beta, Platelet derived growth factor beta polypeptide, Platelet derived growth factor beta polypeptide (oncogene SIS), Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog), Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Platelet-derived growth factor subunit B, Proto-oncogene c-Sis, Simian sarcoma viral (v sis) oncogene homolog, SIS, SSV, v sis platelet derived growth factor beta polypeptide

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Immunofluorescence - Anti-PDGF B Antibody (A286019)

PDGF B expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PDGF B Antibody (A286019) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Flow Cytometry - Anti-PDGF B Antibody (A286019)

PDGF B expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-PDGF B Antibody (A286019) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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