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Anti-PDE5A/PDE5 Antibody (A83168)

This product is identical to Abcam (ab28761). Discover more for less.

Western Blot - Anti-PDE5A Antibody (A83168) Immunofluorescence - Anti-PDE5A Antibody (A83168) Flow Cytometry - Anti-PDE5A Antibody (A83168)
$480
Product Datasheet

Goat polyclonal antibody to PDE5A/PDE5 for ELISA, WB, IF and Flow Cytometry.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

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+1 (314) 370-6046
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Name
Anti-PDE5A/PDE5 Antibody
Description
Goat polyclonal antibody to PDE5A/PDE5.
Applications
ELISA, WB, IF, Flow Cytometry
Dilutions
ELISA: 1:16,000, WB: 0.3-1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C terminal region of human PDE5A/PDE5.
Sequence
C-LINGESGQAKRN
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
95 kDa
Predicted MW
94.8kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-PDE5A Antibody (A83168)Enlarge Image
Figure 1: Western Blot - Anti-PDE5A/PDE5 Antibody (A83168)
HEK293 overexpressing PDE5A (RC218974) and probed with Anti-PDE5A Antibody (A83168) (mock transfection in first lane).
Immunofluorescence - Anti-PDE5A Antibody (A83168)Enlarge Image
Figure 2: Immunofluorescence - Anti-PDE5A/PDE5 Antibody (A83168)
Anti-PDE5A Antibody (A83168) - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing cytoplasmic staining.
Flow Cytometry - Anti-PDE5A Antibody (A83168)Enlarge Image
Figure 3: Flow Cytometry - Anti-PDE5A/PDE5 Antibody (A83168)
Anti-PDE5A Antibody (A83168) - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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