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Anti-PD1 Antibody [EH12.2H7] (APC) (A121900)

Flow Cytometry - Anti-CD279 Antibody [EH12.2H7] (APC) (A121899) - Antibodies.com Flow Cytometry - Anti-CD279 Antibody [EH12.2H7] (APC) (A121900) - Antibodies.com
$430
Product Datasheet

Mouse monoclonal [EH12.2H7] antibody to PD1 (APC) for Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

Freight Charges
$40 to United States
Lead Time
4-6 Business Days

Telephone
+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-PD1 Antibody [EH12.2H7] (APC)
Description
Mouse monoclonal [EH12.2H7] antibody to PD1 (APC).
Specificity
This antibody recognizes an extracellular epitope of CD279, a 55 kDa type I transmembrane protein expressed above all during T cell development, on activated T cells, activated B cells, and activated monocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Unfortunately, this information is considered to be commercially sensitive.
Host
Mouse
Clonality
Monoclonal
Clone ID
EH12.2H7
Isotype
IgG1
Light Chains
kappa
Conjugate

APC

Excitation: 645nm, Emission: 660nm

Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD279, CD279 antigen, hPD 1, hPD l, hPD-1, hSLE1, PD 1, PD-1, PDCD 1, PDCD1, PDCD1_HUMAN, Programmed cell death 1, Programmed cell death 1 protein, Programmed cell death protein 1, Protein PD 1, Protein PD-1, SLEB2, Systemic lupus erythematosus susceptibility 2
Isotype Controls
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Flow Cytometry - Anti-CD279 Antibody [EH12.2H7] (APC) (A121899) - Antibodies.comEnlarge Image
Figure 1: Flow Cytometry - Anti-PD1 Antibody [EH12.2H7] (APC) (A121900)
Flow cytometric analysis of human PHA stimulated peripheral blood mononuclear cells stained with Anti-CD279 Antibody [EH12.2H7] (APC) (10µl reagent per milion cells in 100µl of cell suspension).
Flow Cytometry - Anti-CD279 Antibody [EH12.2H7] (APC) (A121900) - Antibodies.comEnlarge Image
Figure 2: Flow Cytometry - Anti-PD1 Antibody [EH12.2H7] (APC) (A121900)
Separation of human CD297 positive cells (red-filled) from cellular debris (black-dashed) in flow cytometry analysis of human PHA stimulated peripheral blood mononuclear cells using Anti-CD279 Antibody [EH12.2H7] (APC) (10µl reagent per milion cells in 100µl of cell suspension).
Publishing research using Anti-PD1 Antibody [EH12.2H7] (APC) (A121900)? Please let us know so that we can list the citation on this page.
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