Anti-PD-L1 Antibody [ARC2478] (A307692)

Name: Anti-PD-L1 Antibody [ARC2478]
Brand: Antibodies.com
SKU: A307692

Enlarge Image

This product is discontinued

Anti-PD-L1 Antibody [ARC2478] (A307692) has been discontinued and is no longer available.

View all PD-L1 Antibodies.

Specifications

Name

Anti-PD-L1 Antibody [ARC2478]

Description

Rabbit monoclonal [ARC2478] antibody to PD-L1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PD-L1/CD274 (Q9NZQ7).

Sequence

MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2478

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 - 50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

B7 H, B7 H1, B7 homolog 1, B7-H1, B7H, B7H1, CD 274, CD274, CD274 antigen, CD274 molecule, MGC142294, MGC142296, OTTHUMP00000021029, PD L1, PD1L1_HUMAN, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL 1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1, RGD1566211

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

Enlarge Image

Figure 1: Western Blot - Anti-PD-L1 Antibody [ARC2478] (A307692)

Western blot analysis of extracts of U-87MG cells, using Anti-PD-L1 Antibody [ARC2478] (A307692) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Figure 2: Western Blot - Anti-PD-L1 Antibody [ARC2478] (A307692)

Western blot analysis of extracts of various cell lines, using Anti-PD-L1 Antibody [ARC2478] (A307692) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-PD-L1 Antibody [ARC2478] (A307692)

Immunohistochemistry analysis of paraffin-embedded human chronic tonsillitis using Anti-PD-L1 Antibody [ARC2478] (A307692) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-PD-L1 Antibody [ARC2478] (A307692)

Immunohistochemistry analysis of paraffin-embedded human intestinal adenocarcinoma (negative control samples) using Anti-PD-L1 Antibody [ARC2478] (A307692) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Show more images

Publishing research using Anti-PD-L1 Antibody [ARC2478] (A307692)? Please let us know so that we can list the citation on this page.

Alternative products to Anti-PD-L1 Antibody [ARC2478] (A307692)

(5)

A248773

Anti-PD-L1 Antibody [PDL1/2746]

Mouse monoclonal [PDL1/2746] antibody to PD-L1 for ELISA, WB, Flow Cytometry, IF and IHC-P.

(5)

A251953

Anti-PD-L1 Antibody [PDL1/2746] - BSA and Azide free

Mouse monoclonal [PDL1/2746] antibody to PD-L1 for ELISA, WB, Flow Cytometry, IF and IHC-P.

(4)

A323294

Recombinant human monoclonal antibody to PD-L1 for ELISA, FACS, Functional Assay and In Vivo.

(7)

A323290

Atezolizumab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free

Recombinant humanized monoclonal antibody to PD-L1 for ELISA, FACS, Functional Assay and In Vivo.

(3)

A248775

Anti-PD-L1 Antibody [PDL1/4451R]

Recombinant rabbit monoclonal [PDL1/4451R] antibody to PD-L1 for WB, Flow Cytometry, IF and IHC-P.

(5)

A323404

Durvalumab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free

Recombinant human monoclonal antibody to PD-L1 for ELISA, FACS, Functional Assay and In Vivo.

(5)

A82538

Anti-PD-L1 Antibody

Goat polyclonal antibody to PD-L1 for ELISA, WB, IF and Flow Cytometry.

(7)

A93289

Anti-PD-L1 Antibody [ARC5110-01]

Rabbit monoclonal [ARC5110-01] antibody to PD-L1 for WB and IHC.

(3)

A251955

Anti-PD-L1 Antibody [PDL1/4451R] - BSA and Azide free

Recombinant rabbit monoclonal [PDL1/4451R] antibody to PD-L1 for WB, Flow Cytometry, IF and IHC-P.

(4)

A80578

Anti-PD-L1 Antibody

Rabbit polyclonal antibody to PD-L1 for WB and IHC.

(2)

A323472

Garivulimab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free

Recombinant human monoclonal antibody to PD-L1 for ELISA, FACS, Functional Assay and In Vivo.

(2)

A323647

Pacmilimab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free

Recombinant human monoclonal antibody to PD-L1 for ELISA, FACS, Functional Assay and In Vivo.

(5)

A248773

Anti-PD-L1 Antibody [PDL1/2746]

Mouse monoclonal [PDL1/2746] antibody to PD-L1 for ELISA, WB, Flow Cytometry, IF and IHC-P.

(5)

A251953

Anti-PD-L1 Antibody [PDL1/2746] - BSA and Azide free

Mouse monoclonal [PDL1/2746] antibody to PD-L1 for ELISA, WB, Flow Cytometry, IF and IHC-P.

(4)

A323294

Recombinant human monoclonal antibody to PD-L1 for ELISA, FACS, Functional Assay and In Vivo.

(7)

A323290

Atezolizumab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free

Recombinant humanized monoclonal antibody to PD-L1 for ELISA, FACS, Functional Assay and In Vivo.

(3)

A248775

Anti-PD-L1 Antibody [PDL1/4451R]

Recombinant rabbit monoclonal [PDL1/4451R] antibody to PD-L1 for WB, Flow Cytometry, IF and IHC-P.

(5)

A323404

Durvalumab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free

Recombinant human monoclonal antibody to PD-L1 for ELISA, FACS, Functional Assay and In Vivo.

(5)

A82538

Anti-PD-L1 Antibody

Goat polyclonal antibody to PD-L1 for ELISA, WB, IF and Flow Cytometry.

(7)

A93289

Anti-PD-L1 Antibody [ARC5110-01]

Rabbit monoclonal [ARC5110-01] antibody to PD-L1 for WB and IHC.

(3)

A251955

Anti-PD-L1 Antibody [PDL1/4451R] - BSA and Azide free

Recombinant rabbit monoclonal [PDL1/4451R] antibody to PD-L1 for WB, Flow Cytometry, IF and IHC-P.

(4)

A80578

Anti-PD-L1 Antibody

Rabbit polyclonal antibody to PD-L1 for WB and IHC.

(2)

A323472

Garivulimab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free

Recombinant human monoclonal antibody to PD-L1 for ELISA, FACS, Functional Assay and In Vivo.

(2)

A323647

Pacmilimab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free

Recombinant human monoclonal antibody to PD-L1 for ELISA, FACS, Functional Assay and In Vivo.

See all PD-L1 Antibodies

Worldwide Delivery

Live Support

100,000+ Products

100% Guarantee