Anti-PD-L1 Antibody [ARC2478] (A307692)

Name: Anti-PD-L1 Antibody [ARC2478]
Brand: Antibodies.com
SKU: A307692

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This product is discontinued

Anti-PD-L1 Antibody [ARC2478] (A307692) has been discontinued and is no longer available.

View all PD-L1 Antibodies.

Specifications

Name

Anti-PD-L1 Antibody [ARC2478]

Description

Rabbit monoclonal [ARC2478] antibody to PD-L1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PD-L1/CD274 (Q9NZQ7).

Sequence

MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2478

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 - 50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

B7 homolog 1, B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (8)

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Western Blot - Anti-PD-L1 Antibody [ARC2478] (A307692)

Western blot analysis of extracts of U-87MG cells, using Anti-PD-L1 Antibody [ARC2478] (A307692) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Western Blot - Anti-PD-L1 Antibody [ARC2478] (A307692)

Western blot analysis of extracts of various cell lines, using Anti-PD-L1 Antibody [ARC2478] (A307692) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Immunohistochemistry - Anti-PD-L1 Antibody [ARC2478] (A307692)

Immunohistochemistry analysis of paraffin-embedded human chronic tonsillitis using Anti-PD-L1 Antibody [ARC2478] (A307692) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-PD-L1 Antibody [ARC2478] (A307692)

Immunohistochemistry analysis of paraffin-embedded human intestinal adenocarcinoma (negative control samples) using Anti-PD-L1 Antibody [ARC2478] (A307692) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.