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Anti-PD-L1 Antibody (A80578)

Western Blot - Anti-PD-L1 Antibody (A80578) - Antibodies.com Western Blot - Anti-PD-L1 Antibody (A80578) - Antibodies.com Immunohistochemistry - Anti-PD-L1 Antibody (A80578) - Antibodies.com Immunohistochemistry - Anti-PD-L1 Antibody (A80578) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to PD-L1 for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-PD-L1 Antibody
Description
Rabbit polyclonal antibody to PD-L1.
Applications
WB, IHC
Dilutions
WB: 1:100-1:500, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-50 of human PD-L1/CD274 (NP_054862.1).
Sequence
MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
40 - 50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
B7 H, B7 H1, B7 homolog 1, B7-H1, B7H, B7H1, CD 274, CD274, CD274 antigen, CD274 molecule, MGC142294, MGC142296, OTTHUMP00000021029, PD L1, PD1L1_HUMAN, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL 1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1, RGD1566211
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-PD-L1 Antibody (A80578) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-PD-L1 Antibody (A80578)
Western blot analysis of extracts of various cell lines, using Anti-PD-L1 Antibody (A80578) at 1:500 dilution. A-549 cells were treated by IFNG (100 ng/ml) at 37°C for 48 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-PD-L1 Antibody (A80578) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-PD-L1 Antibody (A80578)
Western blot analysis of extracts of Mouse skeletal muscle, using Anti-PD-L1 Antibody (A80578) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-PD-L1 Antibody (A80578) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-PD-L1 Antibody (A80578)
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-PD-L1 Antibody (A80578) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-PD-L1 Antibody (A80578) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-PD-L1 Antibody (A80578)
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-PD-L1 Antibody (A80578) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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