Anti-PD-L1 Antibody (A309668)

Name: Anti-PD-L1 Antibody
Brand: Antibodies.com
SKU: A309668

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This product is discontinued

Anti-PD-L1 Antibody (A309668) has been discontinued and is no longer available.

View all PD-L1 Antibodies.

Specifications

Name

Anti-PD-L1 Antibody

Description

Rabbit polyclonal antibody to PD-L1.

Applications

Dilutions

WB: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PD-L1/CD274.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 - 50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

B7 homolog 1, B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-PD-L1 Antibody (A309668)

Western blot analysis of extracts from normal (control) and PD-L1/CD274 knockout (KO) A-549, A-549+IFNG cells, using Anti-PD-L1 Antibody (A309668) at 1:500 dilution. A-549 cells were treated by IFNG (100 ng/ml) at 37°C for 48 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Western Blot - Anti-PD-L1 Antibody (A309668)

Western blot analysis of extracts of various cell lines, using Anti-PD-L1 Antibody (A309668) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.