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Anti-PD-L1 Antibody [29E.2A3] (PE-Cyanine 7) (A122003)

Flow Cytometry - Anti-CD274 Antibody [29E.2A3] (PE-Cyanine 7) (A122002) - Antibodies.com Flow Cytometry - Anti-CD274 Antibody [29E.2A3] (PE-Cyanine 7) (A122003) - Antibodies.com
$470
Product Datasheet

Mouse monoclonal [29E.2A3] antibody to PD-L1 (PE-Cyanine 7) for Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

Freight Charges
$40 to United States
Lead Time
4-6 Business Days

Telephone
+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-PD-L1 Antibody [29E.2A3] (PE-Cyanine 7)
Description
Mouse monoclonal [29E.2A3] antibody to PD-L1 (PE-Cyanine 7).
Specificity
This antibody recognizes an extracellular epitope of CD274, a 40 kDa type I transmembrane protein expressed by dendritic cells, activated T cells, activated monocytes, and in various tissues, above all in heart and skeletal muscle, placenta and lung, and in many cancer cells, including: T cell lymphomas, melanomas, and glioblastomas.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Full-length human CD274.
Host
Mouse
Clonality
Monoclonal
Clone ID
29E.2A3
Isotype
IgG2b
Light Chains
kappa
Conjugate

PE-Cyanine 7

Excitation: 565nm, Emission: 778nm

Purification
This antibody is conjugated with tandem dye PE-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
B7 H, B7 H1, B7 homolog 1, B7-H1, B7H, B7H1, CD 274, CD274, CD274 antigen, CD274 molecule, MGC142294, MGC142296, OTTHUMP00000021029, PD L1, PD1L1_HUMAN, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL 1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1, RGD1566211
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Flow Cytometry - Anti-CD274 Antibody [29E.2A3] (PE-Cyanine 7) (A122002) - Antibodies.comEnlarge Image
Figure 1: Flow Cytometry - Anti-PD-L1 Antibody [29E.2A3] (PE-Cyanine 7) (A122003)
Flow cytometric analysis of human PHA stimulated peripheral blood mononulcear cells stained with Anti-CD274 Antibody [29E.2A3] (PE-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension).
Flow Cytometry - Anti-CD274 Antibody [29E.2A3] (PE-Cyanine 7) (A122003) - Antibodies.comEnlarge Image
Figure 2: Flow Cytometry - Anti-PD-L1 Antibody [29E.2A3] (PE-Cyanine 7) (A122003)
Separation of human PHA stimulated peripheral blood mononulcear cells stained with Anti-CD274 Antibody [29E.2A3] (PE-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension, red-filled) from PHA stimulated peripheral blood mononulcear cells stained with Mouse IgG2b (PE-Cyanine 7) (Isotype Control) (concentration in sample is 1.67µg/ml, same as Anti-CD274 Antibody [29E.2A3] (PE-Cyanine 7) concentration, black-dashed) in flow cytometry analysis of peripheral blood mononulcear cell suspension.
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