PCNA expression in Jurkat cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-PCNA Antibody (A83563) at 0.05µg/ml and detected by chemiluminescence.
PCNA expression in NIH3T3 (A), Mouse Testes (B), and Rat Testis (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-PCNA Antibody (A83563) at 0.03µg/ml and detected by chemiluminescence.
PCNA expression in Pig Spleen lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-PCNA Antibody (A83563) at 0.3µg/ml and detected by chemiluminescence.
PCNA expression in Human Tonsil analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-PCNA Antibody (A83563) at 2.5µg/ml and revealed with alkaline phosphatase (AP).
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