Anti-PCNA Antibody (A83563)

Name: Anti-PCNA Antibody
Brand: Antibodies.com
SKU: A83563
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to PCNA for ELISA, WB and IHC.

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Alternative Formats and ConjugatesAlternative Formats

Biotin

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Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Nuclear Loading Control

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Anti-PCNA Antibody

Rabbit polyclonal antibody to PCNA for WB, IP and ChIP.

Specifications

Name

Anti-PCNA Antibody

Description

Goat polyclonal antibody to PCNA.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:128,000, WB: 0.03-0.1µg/ml, IHC: 2.5µg/ml

Reactivity

Human, Mouse, Rat, Porcine

Immunogen

Synthetic peptide corresponding to the internal region, amino acids 111-122, of human PCNA.

Sequence

C-EAPNQEKVSDYE

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

30 kDa

Predicted MW

28.8kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ATLD2, cb16, Cyclin, DNA polymerase delta auxiliary protein, etID36690.10, fa28e03, fb36g03, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, Pcna/cyclin, PCNAR, PCNA_HUMAN, Polymerase delta accessory protein, Proliferating cell nuclear antigen, wu:fa28e03, wu:fb36g03

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-PCNA Antibody (A83563)

PCNA expression in Jurkat cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-PCNA Antibody (A83563) at 0.05µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-PCNA Antibody (A83563)

PCNA expression in NIH3T3 (A), Mouse Testes (B), and Rat Testis (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-PCNA Antibody (A83563) at 0.03µg/ml and detected by chemiluminescence.

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Figure 3: Western Blot - Anti-PCNA Antibody (A83563)

PCNA expression in Pig Spleen lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-PCNA Antibody (A83563) at 0.3µg/ml and detected by chemiluminescence.

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Figure 4: Immunohistochemistry - Anti-PCNA Antibody (A83563)

PCNA expression in Human Tonsil analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-PCNA Antibody (A83563) at 2.5µg/ml and revealed with alkaline phosphatase (AP).

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