Anti-PCK2 Antibody (A306806)

Name: Anti-PCK2 Antibody
Brand: Antibodies.com
SKU: A306806
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit polyclonal antibody to PCK2 for WB, IHC, ICC/IF and IP.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-PCK2 Antibody

Description

Rabbit polyclonal antibody to PCK2.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IHC: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PEPCK/PCK2 (NP_004554.2).

Sequence

MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVQLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

71 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EC 4.1.1.32, GTP mitochondrial precursor, HGNC:8725, mitochondrial, Mitochondrial phosphoenolpyruvate carboxykinase 2, OTTHUMP00000164700, PCKGM_HUMAN, PE, PEP carboxykinase, PEPCK, PEPCK deficiency mitochondrial, PEPCK M, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase 2 (mitochondrial), Phosphoenolpyruvate carboxykinase 2 mitochondrial, Phosphoenolpyruvate carboxykinase [GTP], Phosphoenolpyruvate carboxylase, Phosphopyruvate carboxylase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

Enlarge Image

Figure 1: Western Blot - Anti-PCK2 Antibody (A306806)

Western blot analysis of various lysates, using Anti-PCK2 Antibody (A306806) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

Enlarge Image

Figure 2: Western Blot - Anti-PCK2 Antibody (A306806)

Western blot analysis of extracts from wild type(WT) and PEPCK/PCK2 Rabbit polyclonal antibody knockout (KO) 293T cells, using Anti-PCK2 Antibody (A306806) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-PCK2 Antibody (A306806)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-PCK2 Antibody (A306806) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-PCK2 Antibody (A306806)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-PCK2 Antibody (A306806) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.