Anti-PCBP1 Antibody (A306708)

Name: Anti-PCBP1 Antibody
Brand: Antibodies.com
SKU: A306708
Price: 470.0 USD
Availability: InStock

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$470

rabbit polyclonal antibody to PCBP1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Knockout Validated Antibody

Specifications

Name

Anti-PCBP1 Antibody

Description

rabbit polyclonal antibody to PCBP1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human hnRNP E1/PCBP1 (NP_006187.2).

Sequence

LRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDR

Host

rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein X, hnRNP E1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX, nucleic acid binding protein sub 2.3, Nucleic acid- binding protein SUB2.3, Nucleic acid-binding protein SUB2.3, PCBP1_HUMAN, poly(rC) binding protein 1, Poly(rC)-binding protein 1, rCbinding protein 1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PCBP1 Antibody (A306708)

Western blot analysis of extracts of various cell lines, using Anti-PCBP1 Antibody (A306708) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-PCBP1 Antibody (A306708)

Western blot analysis of extracts from wild type(WT) and hnRNP E1/PCBP1 knockout (KO) HeLa cells, using Anti-PCBP1 Antibody (A306708) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-PCBP1 Antibody (A306708)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-PCBP1 Antibody (A306708) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PCBP1 Antibody (A306708)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-PCBP1 Antibody (A306708) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.