Anti-PBR Antibody (A84643)

Name: Anti-PBR Antibody
Brand: Antibodies.com
SKU: A84643
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to PBR for ELISA, WB, IHC and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PBR Antibody

Description

Goat polyclonal antibody to PBR.

Applications

ELISA, WB, IHC, Flow Cytometry

Dilutions

ELISA: 1:128,000, WB: 0.01-0.03µg/ml, IHC: 5-10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Mouse

Immunogen

Synthetic peptide corresponding to the C terminal region of mouse PBR.

Sequence

C-RDNSGRRGGSRLPE

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

16-17 kDa

Predicted MW

18.8kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Benzodiazapine receptor (peripheral), Benzodiazepine peripheral binding site, BPBS, BZRP, DBI, IBP, Isoquinoline carboxamide-binding protein, MBR, mDRC, Mitochondrial benzodiazepine receptor, PBS, Peripheral benzodiazepine receptor, Peripheral benzodiazepine receptor-related protein, Peripheral type benzodiazepine receptor, Peripheral-type benzodiazepine receptor, pk18, PKBS, PTBR, Ptbzr, PTBZR02, RATPTBZR02, Translocator protein, translocator protein (18kDa), Tspo, Tspo1, TSPOA_HUMAN

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-PBR Antibody (A84643)

PBR expression in Mouse Kidney lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PBR Antibody (A84643) at 0.01µg/ml and detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-PBR Antibody (A84643)

PBR expression in Mouse Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-PBR Antibody (A84643) at 10µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 3: Immunohistochemistry - Anti-PBR Antibody (A84643)

Negative control for PBR expression in Mouse Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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Figure 4: Flow Cytometry - Anti-PBR Antibody (A84643)

PBR expression in NIH3T3 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-PBR Antibody (A84643) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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