Anti-PBR Antibody (A83177)

Name: Anti-PBR Antibody
Brand: Antibodies.com
SKU: A83177
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to PBR for ELISA, WB, IHC, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-PBR Antibody

Description

Goat polyclonal antibody to PBR.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Dilutions

ELISA: 1:128,000, WB: 0.1-0.3µg/ml, IHC: 2-4µg/ml, 5µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the C terminal region of human PBR.

Sequence

C-RDNHGWRGGRRLPE

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

17 kDa

Predicted MW

18.8kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Benzodiazapine receptor (peripheral), Benzodiazepine peripheral binding site, BPBS, BZRP, DBI, IBP, Isoquinoline carboxamide-binding protein, MBR, mDRC, Mitochondrial benzodiazepine receptor, PBS, Peripheral benzodiazepine receptor, Peripheral benzodiazepine receptor-related protein, Peripheral type benzodiazepine receptor, Peripheral-type benzodiazepine receptor, pk18, PKBS, PTBR, Ptbzr, PTBZR02, RATPTBZR02, Translocator protein, translocator protein (18kDa), Tspo, Tspo1, TSPOA_HUMAN

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-PBR Antibody (A83177)

PBR expression in A431 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PBR Antibody (A83177) at 0.3µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-PBR Antibody (A83177)

PBR expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PBR Antibody (A83177) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-PBR Antibody (A83177)

PBR expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PBR Antibody (A83177) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-PBR Antibody (A83177)

PBR expression in MCF7 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-PBR Antibody (A83177) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.