Figure 1: Western Blot - Anti-PAX8 Antibody (A83481)
PAX8 expression in Human Kidney (A) and HeLa cell (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PAX8 Antibody (A83481) at 1µg/ml and detected by chemiluminescence.
PAX8 expression in Human Kidney Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-PAX8 Antibody (A83481) at 5µg/ml and revealed with alkaline phosphatase (AP).
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