Anti-Parvalbumin Antibody (A85316)

Adult rat cerebellum floating section was stained with Anti-Parvalbumin Antibody (1:2,500 | green). Parvalbumin is prominently expressed in the dendrites and perikarya of Purkinje cells and the molecular layer interneurons. Blue is a DNA stain.

Immunofluorescent analysis of a rat cerebellum section stained with Anti-Parvalbumin Antibody (1:2,000 | green) and Anti-GFAP Antibody (A85422 | 1:500 | red). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free floating sections were stained with above antibodies. The Anti-Parvalbumin Antibody labels the perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The Anti-GFAP Antibody stains the processes of Bergmann glia and astrocytes.

Western blot analysis of skeletal muscle lysates and His-tagged recombinant human proteins using Anti-Parvalbumin Antibody (1:2,000 | green): [1] protein standard (red), [2] mouse muscle, [3] recombinant parvalbumin, [4] recombinant calretinin and [5] recombinant calbindin. Band at 12kDa in muscle lysate is native parvalbumin and 18kDa band in the next lane is His-tagged recombinant parvalbumin. The Anti-Parvalbumin Antibody binds parvalbumin strongly but is not cross-reactive with the closely related calcium binding proteins calretinin or calbindin.