Anti-PARP1 Antibody (A11205)

Name: Anti-PARP1 Antibody
Brand: Antibodies.com
SKU: A11205
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to PARP1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-PARP1 Antibody

Description

Rabbit polyclonal antibody to PARP1.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PARP1 (NP_001609.2).

Sequence

PGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

89 kDa / 113 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADP-ribosyltransferase diphtheria toxin-like 1, ADP-ribosyltransferase diphtheria toxin-like 10, ADPRT, ADPRT 1, ARTD1, ARTD10, DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PARP-10, PARP10, Poly [ADP-ribose] polymerase 1, Poly [ADP-ribose] polymerase 10, Poly[ADP-ribose] synthase 1, PPOL, Protein mono-ADP-ribosyltransferase PARP10, Protein poly-ADP-ribosyltransferase PARP1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-PARP1 Antibody (A11205)

Western blot analysis of extracts of various cell lines, using Anti-PARP1 Antibody (A11205) at 1:3,000 dilution. HeLa cells were treated by staurosporine(1 uM) for 3 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-PARP1 Antibody (A11205)

Western blot analysis of extracts of various cell lines, using Anti-PARP1 Antibody (A11205) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Immunofluorescence - Anti-PARP1 Antibody (A11205)

Confocal immunofluorescence analysis of U-2 OS cells using Anti-PARP1 Antibody (A11205) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).

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