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Anti-PARK7 Antibody (A83672)

Western Blot - Anti-PARK7 Antibody (A83672) - Antibodies.com Western Blot - Anti-PARK7 Antibody (A83672) - Antibodies.com Immunofluorescence - Anti-PARK7 Antibody (A83672) - Antibodies.com Immunohistochemistry - Anti-PARK7 Antibody (A83672) - Antibodies.com Immunohistochemistry - Anti-PARK7 Antibody (A83672) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to PARK7 for ELISA, WB, IF and IHC.

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Alternative Formats and ConjugatesAlternative Formats

Freight Charges
$40 to United States
Lead Time
3-5 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-PARK7 Antibody
Description
Goat polyclonal antibody to PARK7.
Applications
ELISA, WB, IF, IHC
Dilutions
ELISA: 1:128,000, WB: 0.05-0.1µg/ml, IHC: 7µg/ml, IF: 5µg/ml
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide corresponding to the C terminal region of human PARK7.
Sequence
C-AAQVKAPLVLKD
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
24 kDa
Predicted MW
19.9kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CAP1, DJ-1, DJ1, DJ1 protein, Epididymis secretory sperm binding protein Li 67p, FLJ27376, FLJ34360, FLJ92274, HEL S 67p, Oncogene DJ1, OTTHUMP00000001348, OTTHUMP00000001349, OTTHUMP00000001350, OTTHUMP00000001351, PARK7/DJ1, PARK7_HUMAN, Parkinson disease (autosomal recessive, early onset) 7, Parkinson disease protein 7, Parkinson protein 7, Protein DJ-1, SP22
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-PARK7 Antibody (A83672) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-PARK7 Antibody (A83672)
PARK7 expression in HeLa (A) and Jurkat (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PARK7 Antibody (A83672) at 0.001µg/ml and detected by chemiluminescence.
Western Blot - Anti-PARK7 Antibody (A83672) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-PARK7 Antibody (A83672)
PARK7 expression in Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E), and Rat Brain (F) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PARK7 Antibody (A83672) at 0.001µg/ml and detected by chemiluminescence.
Immunofluorescence - Anti-PARK7 Antibody (A83672) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-PARK7 Antibody (A83672)
PARK7 expression in MCF7 (A) and HeLa (B) cells analyzed by immunofluorescence. Staining was performed with Anti-PARK7 Antibody (A83672) at 5µg/ml and detected with Rabbit Anti-goat IgG-Alexa Fluor 488 secondary antibody at 1:1000 with DAPI nuclear counterstaining.
Immunohistochemistry - Anti-PARK7 Antibody (A83672) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-PARK7 Antibody (A83672)
PARK7 expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-PARK7 Antibody (A83672) at 7µg/ml and revealed with horseradish peroxidase (HRP).
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