Anti-PAR2 Antibody [ARC1246] (A306265)

Name: Anti-PAR2 Antibody [ARC1246]
Brand: Antibodies.com
SKU: A306265
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1246] antibody to PAR2 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PAR2 Antibody [ARC1246]

Description

Rabbit monoclonal [ARC1246] antibody to PAR2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 298-397 of human PAR2 (P55085).

Sequence

ICFTPSNLLLVVHYFLIKSQGQSHVYALYIVALCLSTLNSCIDPFVYYFVSHDFRDHAKNALLCRSVRTVKQMQVSLTSKKHSRKSSSYSSSSTTVKTSY

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1246

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Coagulation factor II receptor-like 1, F2RL1, G-protein coupled receptor 11, GPR11, PAR-2, Proteinase-activated receptor 2, Thrombin receptor-like 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-PAR2 Antibody [ARC1246] (A306265)

Western blot analysis of extracts of various cell lines, using Anti-PAR2 Antibody [ARC1246] (A306265) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Western Blot - Anti-PAR2 Antibody [ARC1246] (A306265)

Western blot analysis of extracts of Rat large intestine, using Anti-PAR2 Antibody [ARC1246] (A306265) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Immunohistochemistry - Anti-PAR2 Antibody [ARC1246] (A306265)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PAR2 Antibody [ARC1246] (A306265) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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