Anti-pan Cytokeratin Antibody [PCK/3150] (A250964)

Mouse monoclonal [PCK/3150] antibody to pan Cytokeratin for Flow Cytometry, IF and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-pan Cytokeratin Antibody [PCK/3150]
Description
Mouse monoclonal [PCK/3150] antibody to pan Cytokeratin.
Specificity
This antibody demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, this antibody is reactive with most epithelial types, including: bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, this antibody is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. This antibody is negative in certain normal tissues, including: brain, lymphocytes, all cells of hematolymphoid origin, muscle, brain, nerves, endothelium, and in certain tumors including: most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET) / Ewings, and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.
Applications
Flow Cytometry, IF, IHC-P
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Canine, Feline
Immunogen
Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
PCK/3150
Isotype
IgG2a
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
40-67 kDa (Multiple)
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-pan Cytokeratin Antibody [PCK/3150] - BSA and Azide free (A254144).
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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