Rabbit polyclonal antibody to PAK5 / 6 (Phospho-Ser602 / Ser560)
Specificity
The antibody detects endogenous levels of PAK5 / 6 only when phosphorylated at serine 602 / 560.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of Serine 602 / 560(R-K-S(p)-L-V) derived from Human PAK5 / 6.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HeLa cells (Lane 2), using PAK5/6 (Phospho-Ser602/Ser560) Antibody #11812. The lane on the left is treated with antigen-specific peptide.
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