Anti-PAK1 / PAK2 / PAK3 (Phospho-Thr423 / Thr402 / Thr421) Antibody (A51402)

Name: Anti-PAK1 / PAK2 / PAK3 (Phospho-Thr423 / Thr402 / Thr421) Antibody
Brand: Antibodies.com
SKU: A51402
Price: 265.0 USD
Availability: InStock

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$265

Rabbit polyclonal antibody to PAK1 / PAK2 / PAK3 (Phospho-Thr423 / Thr402 / Thr421) for IHC and IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-PAK1 / PAK2 / PAK3 (Phospho-Thr423 / Thr402 / Thr421) Antibody

Description

Rabbit polyclonal antibody to PAK1 / PAK2 / PAK3 (Phospho-Thr423 / Thr402 / Thr421)

Specificity

The Antibody detects endogenous level of PAK1 / PAK2 / PAK3 only when phosphorylated at threonine 423 / 402 / 421.

Applications

IHC, IF

Dilutions

Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence around phosphorylation site of threonine 423 / 402 / 421 (R-S-T(p)-M-V) derived from Human PAK1 / PAK2 / PAK3.

Host

Rabbit

Clonality

Polyclonal

Conjugate

Unconjugated

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1.0mg / ml

Predicted MW

61-67 (PAK2) 68-74 (PAK1 / 3)kd

Formulation

Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Synonyms

ADRB2, Alpha PAK, Alpha-PAK, MGC130000, MGC130001, p21 activated kinase 1, p21 protein (Cdc42/Rac) activated kinase 1, p21-activated kinase 1, p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related), p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), p65 PAK, p65-PAK, P68-PAK, PAK alpha, PAK-1, PAK1_HUMAN, Paka, PAKalpha, Protein kinase MUK2, Rac/p21-activated kinase, Serine/threonine-protein kinase PAK 1, STE20 homolog yeast

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Immunohistochemistry - Anti-PAK1 / PAK2 / PAK3 (Phospho-Thr423 / Thr402 / Thr421) Antibody (A51402)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) Antibody #11165 (left) or the same antibody preincubated with blocking peptide (right).

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Figure 2: Immunofluorescence - Anti-PAK1 / PAK2 / PAK3 (Phospho-Thr423 / Thr402 / Thr421) Antibody (A51402)

Immunofluorescence staining of methanol-fixed Hela cells using PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) Antibody #11165.

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