The antibody recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).

Applications

WB, IHC-P, Flow Cytometry (Intracellular)

Dilutions

Flow Cytometry: 2 µg/ml; Intracellular staining, IHC-P: Positive Control: tonsil, spleen, WB: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which, however, migrates on SDS-PAGE gels anomalously as an 80 kDa molecule.

Reactivity

Human

Cross Reactivity

This antibody does not cross react with Mouse, Rat or Cow.

Immunogen

Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).

Host

Mouse

Clonality

Monoclonal

Clone ID

MEM-255

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

20S PROTEASOME ALPHA SUBUNIT G1, 20S PROTEASOME ALPHA SUBUNIT G1, 20S, 20S PROTEASOME SUBUNIT PAG1, AT2G27020, T20P8.7, T20P8_7

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (1)

Enlarge Image

Figure 1: Immunohistochemistry - Anti-PAG1 Antibody [MEM-255] (A86214)

Immunohistochemistry staining (paraffin-embedded sections) using Anti-PAG Antibody (A86214). Cbp/PAG is expressed in germinal centers of lymph node lymphoid follicle and in follicular lymphoma (it is absent from mantle zone). Cbp/PAG is also expressed more weakly in T cells in tonsil and the thymic medulla.

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