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p63 Cell Based ELISA Kit (A103337)

Western Blot - p63 Cell Based ELISA Kit (CB5522) - Antibodies.com Protocol Diagram - p63 Cell Based ELISA Kit (CB5522) - Antibodies.com Protocol Summary - p63 Cell Based ELISA Kit (CB5522) - Antibodies.com Western Blot - Control Antibody - p63 Cell Based ELISA Kit (CB5522) - Antibodies.com
$550
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$40 to United States
Lead Time
5-8 Business Days

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Name
p63 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This p63 Cell Based ELISA Kit allows for the detection of p63 and the effects that certain stimulation conditions have on p63 expression in different cell lines. Qualitative determination of p63 concentration is achieved by an indirect ELISA format. In essence, the p63 is captured by Anti-p63 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this p63 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
AIS, Amplified in squamous cell carcinoma, B(p51A), B(p51B), Chronic ulcerative stomatitis protein, CUSP, DN p63 alpha 1, DNp63, EEC3, id:ibd3516, Keratinocyte transcription factor, Keratinocyte transcription factor KET, KET, LMS, MGC115972, MGC192897, NBP, OFC8, OTTHUMP00000209732, OTTHUMP00000209733, OTTHUMP00000209734, OTTHUMP00000209735, OTTHUMP00000209737, OTTHUMP00000209738, OTTHUMP00000209739, OTTHUMP00000209740, OTTHUMP00000209741, OTTHUMP00000209742, OTTHUMP00000209743, OTTHUMP00000209744, p40, p51, P51/P63, p53-related protein p63, p53CP, P63_HUMAN, p73H, p73L, RHS, SHFM4, TAp63alpha, TP53CP, TP53L, TP63, TP73L, Transformation related protein 63, Transformation-related protein 63, Trp53rp1, Trp63, Tumor protein 63, Tumor protein p53-competing protein, Tumor protein p53-like, Tumor protein p63, Tumor protein p63 deltaN isoform delta, Tumor protein p73, Tumor protein p73-like
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - p63 Cell Based ELISA Kit (CB5522) - Antibodies.comEnlarge Image
Figure 1: Western Blot - p63 Cell Based ELISA Kit (A103337)
Western blot analysis of extracts from COS7 cells, using Anti-p63 Antibody.
Protocol Diagram - p63 Cell Based ELISA Kit (CB5522) - Antibodies.comEnlarge Image
Figure 2: p63 Cell Based ELISA Kit (A103337)
A diagram showing the protocol for p63 Cell Based ELISA Kit.
Protocol Summary - p63 Cell Based ELISA Kit (CB5522) - Antibodies.comEnlarge Image
Figure 3: p63 Cell Based ELISA Kit (A103337)
A summary of the protocol for p63 Cell Based ELISA Kit.
Western Blot - Control Antibody - p63 Cell Based ELISA Kit (CB5522) - Antibodies.comEnlarge Image
Figure 4: Western Blot - p63 Cell Based ELISA Kit (A103337)
The mouse monoclonal antibody to GAPDH is used as a positive control inp63 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Publishing research using p63 Cell Based ELISA Kit (A103337)? Please let us know so that we can list the citation on this page.

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