Rabbit polyclonal antibody to p62Dok (phospho-Tyr362)
Specificity
The antibody detects endogenous level of p62Dok only when phosphorylated at tyrosine 362.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 362 (P-I-Y(p)-D-E) derived from Human p62Dok.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Predicted MW
62kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
Docking protein 1, Docking protein 1 62kD, DOK 1, DOK1, DOK1_HUMAN, Downstream of tyrosine kinase 1, p62(dok), pp62
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok (Phospho-Tyr362) Antibody #11276 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from JK cells, treated with G-CSF or calf intestinal phosphatase (CIP), using p62Dok (phospho-Tyr362) Antibody #11276.
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