Rabbit polyclonal antibody to p56Dok-2 (Phospho-Tyr299)
Specificity
The antibody detects endogenous level of p56Dok-2 only when phosphorylated at tyrosine 299.
Applications
WB, IHC, IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y(p)-A-V) derived from Human p56Dok-2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Predicted MW
56kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
Docking protein 2, Docking protein 2 56kDa, DOK 2, DOK R, DOK2, DOK2_HUMAN, Downstream of tyrosine kinase 2, OTTHUMP00000161710, OTTHUMP00000224923, p56(dok 2), p56(dok-2), p56DOK, p56dok 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p56Dok-2 (Phospho-Tyr299) Antibody #11278 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from JK cells, treated with G-CSF or calf intestinal phosphatase (CIP), using p56Dok-2 (Phospho-Tyr299) Antibody #11278.
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