Anti-p53 Antibody (A99235)

Name: Anti-p53 Antibody
Brand: Antibodies.com
SKU: A99235
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to p53 for WB, IHC, IF and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

ER Marker Mitochondrial Marker

Specifications

Name

Anti-p53 Antibody

Description

Rabbit polyclonal antibody to p53.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:40000

Reactivity

Human

Immunogen

Synthetic peptide derived from human p53 (amino acids 344-393).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.

Molecular Weight

43kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-p53 Antibody (A99235)

Western blot analysis of lysates from 293 cells, treated with Etoposide 25uM 60' using Anti-p53 Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-p53 Antibody (A99235)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-p53 Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Immunofluorescence - Anti-p53 Antibody (A99235)

Immunofluorescence analysis of HeLa cells using Anti-p53 Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.