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Anti-P4HA2 Antibody (A14543)

Western Blot - Anti-P4HA2 Antibody (A14543) - Antibodies.com Western Blot - Anti-P4HA2 Antibody (A14543) - Antibodies.com Immunofluorescence - Anti-P4HA2 Antibody (A14543) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to P4HA2 for WB and ICC/IF.

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$40 to United States
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5-8 Business Days

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Name
Anti-P4HA2 Antibody
Description
Rabbit polyclonal antibody to P4HA2.
Applications
WB, ICC/IF
Dilutions
WB: 1:100-1:500, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 239-333 of human P4HA2 (NP_004190.1).
Sequence
SHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYERPVDYLPERDVYESLCRGEGVKLTPRRQKRLFCRYHHGNRAPQLLIAPFKEEDEW
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
61 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
2-oxoglutarate-4-dioxygenase subunit alpha-2, 4 PH alpha 2, 4-PH alpha-2, C-P4Halpha(II), collagen prolyl 4-hydroxylase alpha(II), P4HA2_HUMAN, procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), alpha polypeptide II, procollagen proline, 2 oxoglutarate 4 dioxygenase alpha subunit, isoform 2, Procollagen-proline, procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2, Prolyl 4 hydroxylase alpha 2 subunit, Prolyl 4 hydroxylase subunit alpha 2 [Precursor], Prolyl 4-hydroxylase subunit alpha-2, Prolyl 4-hydroxylase, alpha polypeptide II
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-P4HA2 Antibody (A14543) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-P4HA2 Antibody (A14543)
Western blot analysis of extracts of various cell lines, using Anti-P4HA2 Antibody (A14543) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-P4HA2 Antibody (A14543) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-P4HA2 Antibody (A14543)
Western blot analysis of extracts of Rat placenta, using Anti-P4HA2 Antibody (A14543) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunofluorescence - Anti-P4HA2 Antibody (A14543) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-P4HA2 Antibody (A14543)
Immunofluorescence analysis of NIH/3T3 cells using Anti-P4HA2 Antibody (A14543) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
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