Anti-p40-phox Antibody [ARC2553] (A306699)

Name: Anti-p40-phox Antibody [ARC2553]
Brand: Antibodies.com
SKU: A306699
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2553] antibody to p40-phox for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-p40-phox Antibody [ARC2553]

Description

Rabbit monoclonal [ARC2553] antibody to p40-phox.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 240-339 of human NCF4/p40-phox (Q15080).

Sequence

LRCYYYEDTISTIKDIAVEEDLSSTPLLKDLLELTRREFQREDIALNYRDAEGDLVRLLSDEDVALMVRQARGLPSQKRLFPWKLHITQKDNYRVYNTMP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2553

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CGD3, MGC3810, NCF, NCF 4, NCF-4, NCF4, NCF4_HUMAN, Neutrophil cytosol factor 4, Neutrophil cytosolic factor 4, Neutrophil NADPH oxidase factor 4, p40phox, SH3 and PX domain-containing protein 4, SH3PXD4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-p40-phox Antibody [ARC2553] (A306699)

Western blot analysis of extracts of THP-1 cells, using Anti-p40-phox Antibody [ARC2553] (A306699) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-p40-phox Antibody [ARC2553] (A306699)

Western blot analysis of extracts of Mouse thymus, using Anti-p40-phox Antibody [ARC2553] (A306699) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-p40-phox Antibody [ARC2553] (A306699)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-p40-phox Antibody [ARC2553] (A306699) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Western Blot - Anti-p40-phox Antibody [ARC2553] (A306699)

Immunoprecipitation analysis of 300µg extracts of THP-1 cells using 3µg of Anti-p40-phox Antibody [ARC2553] (A306699). This Western blot was performed on the immunoprecipitate using Anti-p40-phox Antibody [ARC2553] (A306699) at a dilution of 1:1000.

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