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Anti-P2X5 Antibody (A80873)

Western Blot - Anti-P2X5 Antibody (A80873) - Antibodies.com Immunofluorescence - Anti-P2X5 Antibody (A80873) - Antibodies.com Immunofluorescence - Anti-P2X5 Antibody (A80873) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to P2X5 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-P2X5 Antibody
Description
Rabbit polyclonal antibody to P2X5.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 341-422 of human P2RX5 (NP_002552.2).
Sequence
KKREFYRDKKYEEVRGLEDSSQEAEDEASGLGLSEQLTSGPGLLGMPEQQELQEPPEAKRGSSSQKGNGSVCPQLLEPHRST
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
115 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ATP receptor, ATP receptor subunit, Ionotropic ATP receptor P2X5, LRH 1, LRH1, Lymphoid restricted histocompatibility antigen 1, MGC47755, P2rx5, P2RX5_HUMAN, P2X purinoceptor 5, P2X5R, Purinergic receptor, Purinergic receptor P2X ligand gated ion channel 5, Purinergic receptor P2X5
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-P2X5 Antibody (A80873) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-P2X5 Antibody (A80873)
Western blot analysis of extracts of various cell lines, using Anti-P2X5 Antibody (A80873) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-P2X5 Antibody (A80873) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-P2X5 Antibody (A80873)
Immunofluorescence analysis of Jurkat cells using Anti-P2X5 Antibody (A80873) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-P2X5 Antibody (A80873) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-P2X5 Antibody (A80873)
Immunofluorescence analysis of mouse spleen using Anti-P2X5 Antibody (A80873) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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