Anti-P2X4 Antibody (A85028)

Name: Anti-P2X4 Antibody
Brand: Antibodies.com
SKU: A85028
Price: 480.0 USD
Availability: InStock

Enlarge Image

$480

Goat polyclonal antibody to P2X4 for ELISA, WB, IF and IHC.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-P2X4 Antibody

Description

Goat polyclonal antibody to P2X4.

Applications

ELISA, WB, IF, IHC

Dilutions

ELISA: 1:16,000, WB: 0.3-1µg/ml, IHC: 5µg/ml, IF: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of mouse P2X4.

Sequence

C-ETDSVVSSVTTKAK

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

48-50 kDa

Predicted MW

43.4kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

AI504491, ATP gated cation channel protein, ATP receptor, AW555605, D5Ertd444e, fi03f02, MGC143034, OTTMUSP00000023741, OTTMUSP00000023745, OTTMUSP00000023746, OTTMUSP00000023747, P2RX4, P2RX4_HUMAN, P2X purinoceptor 4, P2X receptor subunit 4, P2X4R, Purinergic receptor, Purinergic receptor P2X ligand gated ion channel 4, Purinergic receptor P2X4, Purinoceptor P2X4, wu:fi03f02

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

Enlarge Image

Figure 1: Western Blot - Anti-P2X4 Antibody (A85028)

P2X4 expression in A549 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-P2X4 Antibody (A85028) at 0.3µg/ml and detected by chemiluminescence.

Enlarge Image

Figure 2: Immunofluorescence - Anti-P2X4 Antibody (A85028)

P2X4 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-P2X4 Antibody (A85028) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-P2X4 Antibody (A85028)

P2X4 expression in Human Uterus analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-P2X4 Antibody (A85028) at 5µg/ml and revealed with alkaline phosphatase (AP).

Publishing research using Anti-P2X4 Antibody (A85028)? Please let us know so that we can list the citation on this page.