Anti-p23 Antibody [JJ6] (A304948)

Name: Anti-p23 Antibody [JJ6]
Brand: Antibodies.com
SKU: A304948
Price: 420.0 USD
Availability: InStock

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$420

Mouse monoclonal [JJ6] antibody to p23 for WB, IHC, ICC/IF, IP, ELISA and Antibody Microarray.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-p23 Antibody [JJ6]

Description

Mouse monoclonal [JJ6] antibody to p23.

Applications

WB, IHC, ICC/IF, IP, ELISA, Antibody Microarray

Dilutions

WB: 1:2,000 IHC: 1:100, ICC/IF: 1:100

Reactivity

Human, Mouse, Rabbit, Chicken, Guinea Pig, Yeast

Immunogen

Recombinant human full length p23 protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

JJ6

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~23 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Co chaperone p23, cPGES, Cytosolic prostaglandin E synthase, Cytosolic prostaglandin E2 synthase, Hsp90 co chaperone, Hsp90 co-chaperone, Progesterone receptor complex, Progesterone receptor complex p23, Prostaglandin E synthase 3, Prostaglandin E synthase 3 (cytosolic), PTGES 3, PTGES3, Sid 3177, TEBP, TEBP_HUMAN, Telomerase binding protein p23, Telomerase-binding protein p23, Unactive progesterone receptor 23 kD

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunofluorescence - Anti-p23 Antibody [JJ6] (A304948)

Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-p23 Antibody [JJ6] (A304948), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Uniform epidermal staining some evidence of cell-cell borders.

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Figure 2: Western Blot - Anti-p23 Antibody [JJ6] (A304948)

Western blot analysis of human Cell lysates showing detection of p23 protein using Anti-p23 Antibody [JJ6] (A304948) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 3: Immunohistochemistry - Anti-p23 Antibody [JJ6] (A304948)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-p23 Antibody [JJ6] (A304948) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Epidermal, dermal, HF, muscle staining. Bright dermal staining.

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Figure 4: Immunofluorescence - Anti-p23 Antibody [JJ6] (A304948)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-p23 Antibody [JJ6] (A304948), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-p23 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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