Principle of Assay
This kit provides a simple, convenient and rapid method for the determination of the oxidized glutathione (GSSG) content, which is suitable for serum, plasma, animal and plant tissues, blood cells, cells, bacteria, etc. The principle is that reduced glutathione can react with 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) to generate 2-nitro-5-mercaptobenzoic acid, which has a maximum light absorption at 412 nm. Inhibition of the original reduced glutathione in the sample by 2-vinylpyridine, followed by the use of glutathione reductase to reduce GSSG to GSH, thereby allows determination of the content of oxidized glutathione.
