Anti-Osteopontin Antibody [F11-4C11] (A280415)

Anti-Osteopontin Antibody [F11-4C11]

Mouse monoclonal [F11-4C11] antibody to Osteopontin.

This antibody recognises osteopontin (OPN) also known as bone sialoprotein 1, nephropontin secreted phosphoprotein 1 and uropontin. It is an acidic glycoprotein and is a member of the intrinsically disordered class of proteins. OPN plays a role in a multitude of physiological and pathophysiological processes. In inflammation it induces cell adhesion and migration, regulates differentiation of proinflammatory lymphocytes and inhibits apoptosis of inflammatory cells. OPN is expressed by various cell types including bone, neural, epithelial and endothelial cells. It is also expressed by the immune cells T cells, B cells, natural killer cells and macrophages. In its secreted form OPN can be found in biological fluids such as blood, urine, bile and milk. OPN has been found to play a role in cancer progression by enhancing proliferation, survival, motility and invasion of tumor cells in a range of cancers. OPN was upregulated at tumor sites and in blood of the patients, with the level correlating with tumor stage and aggressiveness. High levels of OPN have also been detected in several autoimmune diseases including multiple sclerosis and rheumatoid arthritis. The purified Mouse anti Human osteopontin antibody, clone F11-4C11 (MCA6262GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human osteopontin antibody, clone F03-4E8 (MCA6263B) as the detection antibody.

ELISA: 1 µg/ml

Human

HEK293 derived recombinant human full length osteopontin (Ile17-Asn314).

Mouse

Monoclonal

F11-4C11

IgG1

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human osteopontin antibody, clone F11-4C11, recognizes osteopontin (OPN) also known as bone sialoprotein 1, nephropontin secreted phosphoprotein 1 and uropontin. It is an acidic glycoprotein and is a member of the intrinsically disordered class of proteins (Kurzbach et al. 2013). OPN plays a role in a multitude of physiological and pathophysiological processes. In inflammation it induces cell adhesion and migration, regulates differentiation of proinflammatory lymphocytes and inhibits apoptosis of inflammatory cells (Clemente et al. 2016). OPN is expressed by various cell types including bone, neural, epithelial and endothelial cells. It is also expressed by the immune cells T cells, B cells, natural killer cells and macrophages. In its secreted form OPN can be found in biological fluids such as blood, urine, bile and milk (Castello et al. 2017). OPN has been found to play a role in cancer progression by enhancing proliferation, survival, motility and invasion of tumor cells in a range of cancers. OPN was upregulated at tumor sites and in blood of the patients, with the level correlating with tumor stage and aggressiveness (Castello et al. 2017). High levels of OPN have also been detected in several autoimmune diseases including multiple sclerosis and rheumatoid arthritis (Clemente et al. 2016).The purified Mouse anti Human osteopontin antibody, clone F11-4C11 (MCA6262GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human osteopontin antibody, clone F03-4E8 (MCA6263B) as the detection antibody.

BNSP, Bone sialoprotein 1, BSP I, BSPI, Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, OPN, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, OSTP_HUMAN, PSEC0156, Secreted phosphoprotein 1, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), SPP 1, SPP-1, SPP1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin