Anti-ORP150 Antibody [6E3-2C3] (A304973)

Name: Anti-ORP150 Antibody [6E3-2C3]
Brand: Antibodies.com
SKU: A304973
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [6E3-2C3] antibody to ORP150 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

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Specifications

Name

Anti-ORP150 Antibody [6E3-2C3]

Description

Mouse monoclonal [6E3-2C3] antibody to ORP150.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Full length Human GRP170 Protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

6E3-2C3

Isotype

IgG2b

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~170 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

150 kDa oxygen regulated protein, 150 kDa oxygen-regulated protein, 170 kDa glucose regulated protein, 170 kDa glucose-regulated protein, DKFZp686N08236, FLJ94899, FLJ97572, glucose regulated protein 170, Grp 170, GRP-170, Grp170, HSP 12A, HSP12A, Hyou1, HYOU1_HUMAN, Hypoxia up regulated 1, hypoxia up-regulated 1 precursor, Hypoxia up-regulated protein 1, Hypoxia upregulated 1, Orp 150, ORP-150, oxygen regulated protein (150kD)

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-ORP150 Antibody [6E3-2C3] (A304973)

Western blot analysis of rat liver showing detection of ~170 kDa GRP170 protein using Anti-ORP150 Antibody [6E3-2C3] (A304973) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat liver cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:100 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~170 kDa.

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Figure 2: Western Blot - Anti-ORP150 Antibody [6E3-2C3] (A304973)

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293) lysates showing detection of ~170 kDa GRP170 protein using Anti-ORP150 Antibody [6E3-2C3] (A304973) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK-293 cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:100 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~170 kDa. Other Band(s): 100 kDa.

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Figure 3: Immunofluorescence - Anti-ORP150 Antibody [6E3-2C3] (A304973)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ORP150 Antibody [6E3-2C3] (A304973), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5,000 for 60 minutes room temperature, 5 minutes room temperature. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GRP170 Antibody. (D) Composite.

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