One-step TUNEL Apoptosis Assay Kit (Green Fluorescence) (A319767)

$380
Freight Charges
Lead Time
7-8 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
One-step TUNEL Apoptosis Assay Kit (Green Fluorescence)
Applications
Flow Cytometry, Fluorescence Microscopy, Microplate Fluorescence Assays
Principle of Assay
This kit relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein. This kit provides all the essential components with an optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected at the popular FITC channel ( Ex/Em = 490nm/520 nm).
Platform
Flow Cytometer, Fluorescence Microscope
Sample Type
Cells and tissues
Components
TdT Enzyme, Equilibration Buffer (5X), Label Mix Green, DAPI (500X), BSA Working Solution, TritonX-100 (100%), DNase I (5 U/µl)
Absorption Maxima
490 nm
Emission Maxima
520 nm
Storage
Shipped at 4°C. Store at -20°C. Once opened, refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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