This kit relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein. This kit provides all the essential components with an optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected at the popular FITC channel ( Ex/Em = 490nm/520 nm).
Platform
Flow Cytometer, Fluorescence Microscope
Sample Type
Cells and tissues
Components
TdT Enzyme, Equilibration Buffer (5X), Label Mix Green, DAPI (500X), BSA Working Solution, TritonX-100 (100%), DNase I (5 U/µl)
Absorption Maxima
490 nm
Emission Maxima
520 nm
Storage
Shipped at 4°C. Store at -20°C. Once opened, refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Analysis of HeLa cells treated with 10 U/mL DNase I for 10 minutes using One-step TUNEL Apoptosis Assay Kit (Green Fluorescence) (A319767).
Publishing research using One-step TUNEL Apoptosis Assay Kit (Green Fluorescence) (A319767)? Please let us know so that we can list the citation on this page.
