Anti-OGDH Antibody (A15204)

Name: Anti-OGDH Antibody
Brand: Antibodies.com
SKU: A15204
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to OGDH for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-OGDH Antibody

Description

Rabbit polyclonal antibody to OGDH.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:10-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 869-1023 of human OGDH (NP_002532.2).

Sequence

RSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLTRERKARDMVGQVAITRIEQLSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTISRAKPVWYAGRDPAAAPATGNKKTHLTELQRLLDTAFDLDVFKNFS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

2 oxoglutarate dehydrogenase, 2 oxoglutarate dehydrogenase complex component E1, 2 oxoglutarate dehydrogenase mitochondrial, 2-oxoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase complex component E1, AKGDH, Alpha ketoglutarate dehydrogenase, Alpha-ketoglutarate dehydrogenase, E1k, mitochondrial, ODO1_HUMAN, OGDC, OGDC E1, OGDC-E1, Oxoglutarate (alpha ketoglutarate) dehydrogenase (lipoamide), Oxoglutarate decarboxylase, Oxoglutarate dehydrogenase (lipoamide), Oxoglutarate dehydrogenase (succinyl transferring)

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-OGDH Antibody (A15204)

Western blot analysis of extracts of various cell lines, using Anti-OGDH Antibody (A15204) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-OGDH Antibody (A15204)

Western blot analysis of extracts of LNCaP cells, using Anti-OGDH Antibody (A15204) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-OGDH Antibody (A15204)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-OGDH Antibody (A15204) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-OGDH Antibody (A15204)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-OGDH Antibody (A15204) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.