Figure 1: Western Blot - Anti-OB Cadherin Antibody (A84824)
OB Cadherin expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress MYC tagged OB Cadherin an lysed in RIPA buffer. Primary incubation was performed for 1 hour on 10µg protein per lane with Anti-OB Cadherin Antibody (A84824) at 1µg/ml (Lane A) or Anti-MYC at 1:1000 dilution (Lane C) and detected with chemiluminescence. Control: Mock-transfected HEK293 cells incubated with Anti-OB Cadherin Antibody (A84824) at 1mg/ml (Lane B).
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