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Anti-NUP98 Antibody [13C2 + 21A10] (A383)

Immunofluorescene staining of Nup98 in HeLa cells using 13C2 , 21A10 , or 2H10 monoclonal antibodies. Western blot analysis of Nup98 in HeLa cells with anti-Nup98 antibodies. Immunofluorescene staining of Nup98 in Tetrahymena themophila cells using 13C2 , 21A10 , 2H10 or 414 monoclonal antibodies Detection of MacNup98A of Tetrahymena by Western blotting with monoclonal antibodies 13C2 and 21A10 Immunofluorescene staining of Nup98 in S. pombe cells with 13C2 , 21A10, or 2H10 monoclonal antibodies. Detection of Nup98 in S. pombe cell extracts by Western blotting with monoclonal antibodies 13C2, 21A10 and 2H10. Monoclonal antibodies 13C2 and 21A10 crossreact with multiple nucleoporins of S. cerevisiae. (A) IF staining of S. cereviciae cells using 13C2, 21A10, or 2H10 mAbs: 1/10 dilutions of the supernatants of hybridoma cultures of 13C2 or 21A10 or 10 µg/ml IgG solution of 2H10 were used. Black-and-white images represent fluorescence images obtained with mAb (top) and DAPI (middle). Color images represent merged images of mAb (green) with DAPI (magenta). Dotted lines represent the outlines of cells. Bar, 5 µm. Methods were as described for S. pombe. (B) WB analysis of S. cereviciae cell extract using 13C2 , 21A10, or 2H10 mAbs: 1/10 dilutions of the supernatants of hybridoma culture medium of 13C2 or 21A10 or 2 µg/ml IgG solution of 2H10 were used. Arrows represent the positions of the indicated nucleoporins. Asterisks represent uncharacterized proteins. Methods were as described for S. pombe
$535

Mouse monoclonal (13C2 + 21A10) antibody to NUP98 for WB and ICC/IF.

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Name
Anti-NUP98 Antibody [13C2 + 21A10]
Description
Mouse monoclonal (13C2 + 21A10) antibody to NUP98.
Applications
WB, ICC/IF
Reactivity
Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Tetrahymena
Immunogen
Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of Tetrahymena thermophila.

Peptide 1: 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29

Peptide 2: 646-SNPTQGGGLFGAANPGLGG-664.

Epitope
FGxxN for clone 13C2 and GLF for clone 21A10.
Host
Mouse
Clonality
Monoclonal
Clone ID
13C2 + 21A10
Isotype
IgG1
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
96 kDa nucleoporin, 98 kDa nucleoporin, ADAR2, ADIR2, GLFG-repeat containing nucleoporin, Nuclear pore complex protein Nup96, Nuclear pore complex protein Nup98 Nup96, Nucleoporin 98kD, nucleoporin 98kDa, Nucleoporin Nup96, Nucleoporin Nup98, NUP196, NUP96, Nup98-Nup96, NUP98_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunofluorescene staining of Nup98 in HeLa cells using 13C2 , 21A10 , or 2H10 monoclonal antibodies.Enlarge Image
Figure 1: Anti-NUP98 Antibody [13C2 + 21A10] (A383)
Anti-NUP98 Antibody
Western blot analysis of Nup98 in HeLa cells with anti-Nup98 antibodies.Enlarge Image
Figure 2: Anti-NUP98 Antibody [13C2 + 21A10] (A383)
Anti-NUP98 Antibody
Immunofluorescene staining of Nup98 in Tetrahymena themophila cells using 13C2 , 21A10 , 2H10 or 414 monoclonal antibodiesEnlarge Image
Figure 3: Anti-NUP98 Antibody [13C2 + 21A10] (A383)
Anti-NUP98 Antibody
Detection of MacNup98A of Tetrahymena by Western blotting with monoclonal antibodies 13C2 and 21A10Enlarge Image
Figure 4: Anti-NUP98 Antibody [13C2 + 21A10] (A383)
Anti-NUP98 Antibody
Publishing research using Anti-NUP98 Antibody [13C2 + 21A10] (A383)? Please let us know so that we can list the citation on this page.

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