Anti-NUP50 Antibody (A12363)

Name: Anti-NUP50 Antibody
Brand: Antibodies.com
SKU: A12363
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to NUP50 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-NUP50 Antibody

Description

Rabbit polyclonal antibody to NUP50.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 340-468 of human NUP50 (NP_009103.2).

Sequence

ENDEPPKVVVTEVKEEDAFYSKKCKLFYKKDNEFKEKGIGTLHLKPTANQKTQLLVRADTNLGNILLNVLIPPNMPCTRTGKNNVLIVCVPNPPIDEKNATMPVTMLIRVKTSEDADELHKILLEkDa

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

50 kDa nucleoporin, NPAP60, NPAP60L, Nuclear pore associated protein 60L, Nuclear pore complex protein Nup50, Nuclear pore-associated protein 60 kDa-like, Nucleoporin 50, Nucleoporin 50kDa, Nucleoporin Nup50, NUP50_HUMAN, PRO1146

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-NUP50 Antibody (A12363)

Western blot analysis of extracts of various cell lines, using Anti-NUP50 Antibody (A12363) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-NUP50 Antibody (A12363)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-NUP50 Antibody (A12363) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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