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Anti-Nucleophosmin (phospho Thr199) Antibody (A89605)

Western Blot - Anti-Nucleophosmin (phospho Thr199) Antibody (A89605) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to Nucleophosmin (phospho Thr199) for WB.

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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Nucleophosmin (phospho Thr199) Antibody
Description
Rabbit polyclonal antibody to Nucleophosmin (phospho Thr199).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Rat
Immunogen
A synthetic phosphorylated peptide around T199 of human NPM1 (NP_001032827.1).
Sequence
RDTPA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
38 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
B23, MGC104254, NO38, NPM, NPM1, NPM_HUMAN, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin (nucleolar phosphoprotein B23 numatrin), nucleophosmin nucleoplasmin family member 1, Nucleophosmin/nucleoplasmin family member 1, Numatrin, OTTHUMP00000161024, OTTHUMP00000161025, OTTHUMP00000223397, OTTHUMP00000223398
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Nucleophosmin (phospho Thr199) Antibody (A89605) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Nucleophosmin (phospho Thr199) Antibody (A89605)
Western blot analysis of extracts of various cell lines, using Anti-Nucleophosmin (phospho Thr199) Antibody (A89605) at 1:1,000 dilution or NPM1 antibody (A17983). HeLa cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. PC-12 cells were treated by Nocodazole (1 µg/mL) at 37°C for 16 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Publishing research using Anti-Nucleophosmin (phospho Thr199) Antibody (A89605)? Please let us know so that we can list the citation on this page.

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