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Anti-Nucleophosmin Antibody (A307817)

Western Blot - Anti-Nucleophosmin Antibody (A307817) - Antibodies.com Western Blot - Anti-Nucleophosmin Antibody (A307817) - Antibodies.com Immunohistochemistry - Anti-Nucleophosmin Antibody (A307817) - Antibodies.com Immunohistochemistry - Anti-Nucleophosmin Antibody (A307817) - Antibodies.com Immunohistochemistry - Anti-Nucleophosmin Antibody (A307817) - Antibodies.com Immunofluorescence - Anti-Nucleophosmin Antibody (A307817) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Nucleophosmin for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-Nucleophosmin Antibody
Description
Rabbit polyclonal antibody to Nucleophosmin.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 9-122 of human NPM1 (NP_002511.1).
Sequence
MSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEED
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
38 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
B23, MGC104254, NO38, NPM, NPM1, NPM_HUMAN, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin (nucleolar phosphoprotein B23 numatrin), nucleophosmin nucleoplasmin family member 1, Nucleophosmin/nucleoplasmin family member 1, Numatrin, OTTHUMP00000161024, OTTHUMP00000161025, OTTHUMP00000223397, OTTHUMP00000223398
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Nucleophosmin Antibody (A307817) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Nucleophosmin Antibody (A307817)
Western blot analysis of extracts of various cell lines, using Anti-Nucleophosmin Antibody (A307817) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-Nucleophosmin Antibody (A307817) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Nucleophosmin Antibody (A307817)
Western blot analysis of extracts of various cell lines, using Anti-Nucleophosmin Antibody (A307817) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Nucleophosmin Antibody (A307817) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Nucleophosmin Antibody (A307817)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Nucleophosmin Antibody (A307817) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Nucleophosmin Antibody (A307817) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-Nucleophosmin Antibody (A307817)
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-Nucleophosmin Antibody (A307817) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Publishing research using Anti-Nucleophosmin Antibody (A307817)? Please let us know so that we can list the citation on this page.

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