Anti-Nucleolin Antibody [NCL/902] (CF® 488) (A251141)

Anti-Nucleolin Antibody [NCL/902] (CF® 488)

Mouse monoclonal [NCL/902] antibody to Nucleolin (CF® 488).

This antibody recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Flow Cytometry, IF

Flow Cytometry: 5-10 µl / million cells, IF: 1:50-1:100

Human

This antibody does not cross react with Mouse, Rat, or Bovine.

Recombinant human NCL protein.

Mouse

NCL/902

IgG1

kappa

CF® 488

Excitation: 490nm, Emission: 515nm

Protein A/G chromatography.

100 µg/ml

76 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Store at 4-8°C. Antibody is stable for 24 months.

C23, FLJ45706, MS1116, NCL, Nucl, NUCL_HUMAN, Protein C23

This product is for research use only. It is not intended for diagnostic or therapeutic use.