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Nuclear Factor 1 Cell Based ELISA Kit (A102823)

Western Blot - Nuclear Factor 1 Cell Based ELISA Kit (CB5975) - Antibodies.com Protocol Diagram - Nuclear Factor 1 Cell Based ELISA Kit (CB5975) - Antibodies.com Protocol Summary - Nuclear Factor 1 Cell Based ELISA Kit (CB5975) - Antibodies.com Western Blot - Control Antibody - Nuclear Factor 1 Cell Based ELISA Kit (CB5975) - Antibodies.com
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$40 to United States
Lead Time
5-8 Business Days

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Name
Nuclear Factor 1 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This Nuclear Factor 1 Cell Based ELISA Kit allows for the detection of Nuclear Factor 1 and the effects that certain stimulation conditions have on Nuclear Factor 1 expression in different cell lines. Qualitative determination of Nuclear Factor 1 concentration is achieved by an indirect ELISA format. In essence, the Nuclear Factor 1 is captured by Anti-Nuclear Factor 1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Nuclear Factor 1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
1110019L22Rik, 1500041O16Rik, AA589446, AI746521, CAAT box transcription factor, CCAAT binding transcription factor, CCAAT box binding transcription factor, CCAAT-box-binding transcription factor, CNFI C, CTF, CTF5, MGC137374, MGC20153, NF I, NF I/C, NF-I/C, NF1 C, NF1-C, NF1C, NFI, NFI-C, NFI/C, NFIC, NFIC_HUMAN, Nuclear factor 1 C type, Nuclear factor 1 C-type, Nuclear factor 1/C, Nuclear factor I/C, Nuclear factor I/C (CCAAT binding transcription factor), TGGCA binding protein, TGGCA-binding protein, Transcription factor NFIC
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Nuclear Factor 1 Cell Based ELISA Kit (CB5975) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Nuclear Factor 1 Cell Based ELISA Kit (A102823)
Western blot analysis of extracts from rat brain/HuvEc cells, using Anti-Nuclear Factor 1 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - Nuclear Factor 1 Cell Based ELISA Kit (CB5975) - Antibodies.comEnlarge Image
Figure 2: Nuclear Factor 1 Cell Based ELISA Kit (A102823)
A diagram showing the protocol for Nuclear Factor 1 Cell Based ELISA Kit.
Protocol Summary - Nuclear Factor 1 Cell Based ELISA Kit (CB5975) - Antibodies.comEnlarge Image
Figure 3: Nuclear Factor 1 Cell Based ELISA Kit (A102823)
A summary of the protocol for Nuclear Factor 1 Cell Based ELISA Kit.
Western Blot - Control Antibody - Nuclear Factor 1 Cell Based ELISA Kit (CB5975) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Nuclear Factor 1 Cell Based ELISA Kit (A102823)
The mouse monoclonal antibody to GAPDH is used as a positive control inNuclear Factor 1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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