Anti-NSE Antibody (A85285)

HEK 293 cells stained with Anti-NSE Antibody and Anti-Ubiquitin (A85350). The red channel shows staining with Anti-NSE Antibody which recognizes all of these cells. The green channels shows staining for Anti-Ubiquitin Antibody. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However, all cells that express NSE also express UCHL1.

Immunofluorescent analysis of mixed cortical neuron-glial cell culture from E20 rat stained with Anti-NSE Antibody (A85285), at a dilution of 1:500, in red, and co-stained with Anti-GFAP Antibody (A85307), at a dilution of 1:5,000 in green. The nuclear DNA is visualised in blue using Hoechst staining. The Anti-NSE Antibody (A85285) labels protein expressed in neuronal cells, while the Anti-GFAP Antibody (A85307) stains intermediate filaments in astrocytic and certain other glial cells.

Western blot analysis of different tissue and cell lysates using Anti-NSE Antibody (A85285), at a dilution of 1:5,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3 cells, [7] HEK293 cells, [8] HeLa cells, [9] SH-SY5Y cells, and [10] C6 cells. A single band at about 47 kDa corresponds to the NSE protein, seen only in extracts containing neurons or neuronal lineage cells.

Blot of rat spinal cord probed with Anti-NSE Antibody. The antibody stains a single sharp band corresponding to NSE at ~47 kDa.