Anti-Nrf2 Antibody [ARC50393] (A308758)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Nrf2 Antibody [ARC50393] (A308758) at 1:1,000 dilution. NIH/3T3 cells were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts of C6 cells, using Anti-Nrf2 Antibody [ARC50393] (A308758) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from wild type(WT) and NRF2 knockout (KO) HeLa cells, using Anti-Nrf2 Antibody [ARC50393] (A308758) at 1:1,000 dilution. HeLa cells were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.