Anti-NQO1 Antibody (A82457)

Name: Anti-NQO1 Antibody
Brand: Antibodies.com
SKU: A82457
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to NQO1 for ELISA, WB, IF and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-NQO1 Antibody

Description

Goat polyclonal antibody to NQO1.

Applications

ELISA, WB, IF, IHC

Dilutions

ELISA: 1:64,000, WB: 0.1-0.3µg/ml, IHC: 2-4µg/ml, IF: 10µg/ml

Reactivity

Human, Rat

Immunogen

Synthetic peptide corresponding to the C terminal region of human NQO1.

Sequence

C-SIPTDNQIKARK

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

30 kDa

Predicted MW

30.9kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Azoreductase, Cytochrome b 5 reductase, DHQU, DIA 4, DIA4, Diaphorase (NADH/NADPH), Diaphorase (NADH/NADPH) (cytochrome b 5 reductase), Diaphorase 4, Dioxin inducible 1, DT diaphorase, DT-diaphorase, DTD, Menadione reductase, NAD(P)H dehydrogenase quinone 1, NAD(P)H dehydrogenase [quinone] 1, NAD(P)H menadione oxidoreductase 1 dioxin inducible, NAD(P)H quinone dehydrogenase 1, NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1, NAD(P)H:menadione oxidoreductase 1, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H:quinone oxidoreductase 1, NAD(P)H:quinone oxireductase, NMOR 1, NMOR I, NMOR1, NMORI, NQO 1, NQO1_HUMAN, Phylloquinone reductase, QR 1, QR1, Quinone reductase 1

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-NQO1 Antibody (A82457)

NQO1 expression in U251 (A) and HepG2 (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-NQO1 Antibody (A82457) at 0.3µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-NQO1 Antibody (A82457)

NQO1 expression in Human Kidney (A), Lung (B) and Duodenum (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-NQO1 Antibody (A82457) at 0.3µg/ml and detected by chemiluminescence.

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Figure 3: Western Blot - Anti-NQO1 Antibody (A82457)

NQO1 expression in Rat Kidney (A), Lung (B) and Duodenum (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-NQO1 Antibody (A82457) at 0.3µg/ml and detected by chemiluminescence.

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Figure 4: Immunohistochemistry - Anti-NQO1 Antibody (A82457)

NQO1 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-NQO1 Antibody (A82457) at 2µg/ml and revealed with alkaline phosphatase (AP).