This antibody recognises Notch 2, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. Signaling through Notch 2 has been implicated in the development of marginal zone B cells (MZB), the sensitization of endothelial cells to apoptosis, and the regulation of the expression of CD23 in B-cell lymphocytic leukemia (B-CLL). There is a negative correlation between Notch 2 expression and the grade of human breast cancer.Hamster anti Mouse Notch 2 antibody, clone HMN2-35 cross-reacts with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry.
Applications
Flow Cytometry
Dilutions
Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Reactivity
Mouse, Rat
Immunogen
Mouse Notch 2-Fc fusion protein.
Host
Hamster
Clonality
Monoclonal
Clone ID
HMN2-35
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Hamster anti Mouse Notch 2 antibody, clone HMN2-35 recognizes Notch 2, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. Signaling through Notch 2 has been implicated in the development of marginal zone B cells (MZB), the sensitization of endothelial cells to apoptosis, and the regulation of the expression of CD23 in B-cell lymphocytic leukemia (B-CLL). There is a negative correlation between Notch 2 expression and the grade of human breast cancer.Hamster anti Mouse Notch 2 antibody, clone HMN2-35 cross-reacts with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry.