Anti-Notch1 Antibody [ARC0285] (A306313)

Name: Anti-Notch1 Antibody [ARC0285]
Brand: Antibodies.com
SKU: A306313
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0285] antibody to Notch1 for WB and IP.

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$40 to United States

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5-8 Business Days

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Neuroepithelial Marker

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Anti-Notch1 Antibody [mN1A]

Mouse monoclonal [mN1A] antibody to Notch1 for Flow Cytometry, IP, WB, IHC-Fr and ICC.

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Anti-Notch1 Antibody [mN1A]

Mouse monoclonal [mN1A] antibody to Notch1 for Flow Cytometry, IP, WB, IHC-Fr and ICC.

Specifications

Name

Anti-Notch1 Antibody [ARC0285]

Description

Rabbit monoclonal [ARC0285] antibody to Notch1.

Applications

WB, IP

Dilutions

WB: 1:500-1:2,000, IP: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 2456-2555 of human Notch1 (P46531).

Sequence

ILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0285

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

125 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

9930111A19Rik, AOS5, AOVD1, hN1, Lin-12, LIN12, Mis6, Motch A, mT14, Neurogenic locus notch homolog protein 1, Neurogenic locus notch protein homolog, NICD, NOTC1_HUMAN, NOTCH, Notch 1, Notch 1 intracellular domain, Notch gene homolog 1 (Drosophila), Notch homolog 1, translocation-associated (Drosophila), NOTCH, Drosophila, homolog of, 1, TAN1, Translocation associated notch homolog, Translocation associated notch protein TAN 1, Translocation-associated notch protein TAN-1, xotch

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Notch1 Antibody [ARC0285] (A306313)

Western blot analysis of extracts of various cell lines, using Anti-Notch1 Antibody [ARC0285] (A306313) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Western Blot - Anti-Notch1 Antibody [ARC0285] (A306313)

Immunoprecipitation analysis of 200µg extracts of 293T cells using 3µg of Anti-Notch1 Antibody [ARC0285] (A306313). This Western blot was performed on the immunoprecipitate using Anti-Notch1 Antibody [ARC0285] (A306313) at a dilution of 1:1000.

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