Anti-Notch1 Antibody (A286055)

Name: Anti-Notch1 Antibody
Brand: Antibodies.com
SKU: A286055
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to Notch1 for ELISA, IF and IHC.

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Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Neuroepithelial Marker

Highlighted products for Anti-Notch1 Antibody

A86356

8 Citations

Anti-Notch1 Antibody [mN1A]

Mouse monoclonal [mN1A] antibody to Notch1 for Flow Cytometry, IP, WB, IHC-Fr and ICC.

A86356

8 Citations

Anti-Notch1 Antibody [mN1A]

Mouse monoclonal [mN1A] antibody to Notch1 for Flow Cytometry, IP, WB, IHC-Fr and ICC.

Specifications

Name

Anti-Notch1 Antibody

Description

Goat polyclonal antibody to Notch1.

Applications

ELISA, IF, IHC

Dilutions

ELISA: 1:8,000, IHC: 5µg/ml, IF: 5µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region (near N terminus), amino acids 171-182, of human Notch1.

Sequence

HGPTCRQDVNEC

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

9930111A19Rik, AOS5, AOVD1, hN1, Lin-12, LIN12, Mis6, Motch A, mT14, Neurogenic locus notch homolog protein 1, Neurogenic locus notch protein homolog, NICD, NOTC1_HUMAN, NOTCH, Notch 1, Notch 1 intracellular domain, Notch gene homolog 1 (Drosophila), Notch homolog 1, translocation-associated (Drosophila), NOTCH, Drosophila, homolog of, 1, TAN1, Translocation associated notch homolog, Translocation associated notch protein TAN 1, Translocation-associated notch protein TAN-1, xotch

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Immunofluorescence - Anti-Notch1 Antibody (A286055)

Notch1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Notch1 Antibody (A286055) at 5µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 5µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Immunohistochemistry - Anti-Notch1 Antibody (A286055)

Notch1 expression in Human Thyroid analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Notch1 Antibody (A286055) at 5µg/ml and revealed with alkaline phosphatase (AP).

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