Anti-Notch1 Antibody (A15817)

Name: Anti-Notch1 Antibody
Brand: Antibodies.com
SKU: A15817
Price: 335.0 USD
Availability: InStock

Enlarge Image

$335

Rabbit polyclonal antibody to Notch1 for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Tags:

Neuroepithelial Marker

Highlighted products for Anti-Notch1 Antibody

A86356

8 Citations

Anti-Notch1 Antibody [mN1A]

Mouse monoclonal [mN1A] antibody to Notch1 for Flow Cytometry, IP, WB, IHC-Fr and ICC.

A86356

8 Citations

Anti-Notch1 Antibody [mN1A]

Mouse monoclonal [mN1A] antibody to Notch1 for Flow Cytometry, IP, WB, IHC-Fr and ICC.

Specifications

Name

Anti-Notch1 Antibody

Description

Rabbit polyclonal antibody to Notch1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1567-1729 of human Notch1 (NP_060087.3).

Sequence

ERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGREEELRKHPIKRAAEGWAAPDALLGQVKASLLPGGSEGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

300 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

9930111A19Rik, AOS5, AOVD1, hN1, Lin-12, LIN12, Mis6, Motch A, mT14, Neurogenic locus notch homolog protein 1, Neurogenic locus notch protein homolog, NICD, NOTC1_HUMAN, NOTCH, Notch 1, Notch 1 intracellular domain, Notch gene homolog 1 (Drosophila), Notch homolog 1, translocation-associated (Drosophila), NOTCH, Drosophila, homolog of, 1, TAN1, Translocation associated notch homolog, Translocation associated notch protein TAN 1, Translocation-associated notch protein TAN-1, xotch

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (11)

Enlarge Image

Figure 1: Western Blot - Anti-Notch1 Antibody (A15817)

Western blot analysis of extracts of Rat brain cells, using Anti-Notch1 Antibody (A15817) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Enlarge Image

Figure 2: Western Blot - Anti-Notch1 Antibody (A15817)

Western blot analysis of extracts of various cell lines, using Anti-Notch1 Antibody (A15817) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-Notch1 Antibody (A15817)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-Notch1 Antibody (A15817) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-Notch1 Antibody (A15817)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Notch1 Antibody (A15817) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.