Anti-NMDAR2A Antibody [S327-95] (A304974)

Name: Anti-NMDAR2A Antibody [S327-95]
Brand: Antibodies.com
SKU: A304974
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S327-95] antibody to NMDAR2A for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-NMDAR2A Antibody [S327-95]

Description

Mouse monoclonal [S327-95] antibody to NMDAR2A.

Specificity

This antibody does not react with NR2B.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A/NR2A.

Host

Mouse

Clonality

Monoclonal

Clone ID

S327-95

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~170 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EPND, FESD, GluN2A, Glutamate receptor, Glutamate receptor ionotropic N methyl D aspartate 2A, Glutamate [NMDA] receptor subunit epsilon-1, GRIN 2A, GRIN2A, hNR2A, LKS, N methyl D aspartate receptor channel, subunit epsilon 1, N Methyl D Aspartate Receptor Subtype 2A, N methyl D aspartate receptor subunit 2A, N-methyl D-aspartate receptor subtype 2A, NMDA receptor subtype 2A, NMDAR 2A, NMDE1_HUMAN, NR2A, OTTHUMP00000160135, OTTHUMP00000174531

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-NMDAR2A Antibody [S327-95] (A304974)

Western blot analysis of Monkey COS cells transfected with GFP-tagged NR2A showing detection of ~170 kDa GluN2A/NR2A protein using Anti-NMDAR2A Antibody [S327-95] (A304974) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NR2A. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~170 kDa. Other Band(s): 100 kDa.

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Figure 2: Immunofluorescence - Anti-NMDAR2A Antibody [S327-95] (A304974)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-NMDAR2A Antibody [S327-95] (A304974), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cell Membrane, Cell Junction. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluN2A/NR2A Antibody. (D) Composite.

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Figure 3: Immunofluorescence - Anti-NMDAR2A Antibody [S327-95] (A304974)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-NMDAR2A Antibody [S327-95] (A304974), at 1:200 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2A/NR2A Antibody (D) Composite.

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